Reversed-Phase-High Performance Liquid Chromatography

An efficient method that is frequently used for glycan profiling is RP-HPLC. This technique allows for the efficient and quick separation of carbohydrates, glycopeptides, and glycoproteins, which may then be further characterized. Glycoprotein analysis is a long-standing area of expertise for our skilled staff. We are certain that we can provide a comprehensive range of glycoprotein analysis services at the most affordable and high-caliber costs. Thus far, we have provided services to hundreds of clients, receiving their unwavering appreciation and recognition in a matter of years.

Peptides, proteins, and glycoproteins can all be analyzed and purified using the widely used RP-HPLC technique. The RP-HPLC separation of p-aminobenzoic ethyl ester derivatives of neutral and amino sugars released from glycoproteins can be used to assess the composition and amount of monosaccharides. Furthermore, a study has demonstrated that the RP-HPLC method, which is superior to chromatographic and/or enrichment approaches like HILIC and lectin affinity, has the best coverage for glycopeptide analysis based on the number of detected glycosylation sites and has identified glycopeptides in three model glycoproteins.